Product Name :
Restriction enzyme FokI(Internal) Rabbit Polyclonal Antibody
Predicted band size :
Observed band size :
Synonyms:
Function :
The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5′-GGATG-3′:3′-CATCC-5′ recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.
Antibody Type:
Rabbit Polyclonal Antibody
Immunogen :
Synthetic peptide within Planomicrobium okeanokoites fokIR aa 444-456.
Species reactivity:
Planomicrobium okeanokoites
Verify application:
WB
Coupling :
unconjugated
Clone number:
RRID:
AB_3073314
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.Gaboxadol (hydrochloride) structure Aliquot store at -20℃ or -80℃.Ammonium iron(III) citrate uses Avoid repeated freeze / thaw cycles.PMID:33382524
Storage buffer solution:
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Immunogen affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 66 kDa
Positive control :
Purified restriction enzyme FokI